Abstract:
Seeds are reservoirs of beneficial microbes that shape early plant development. However, their functional roles and mechanisms remain poorly understood. We isolated 23 SEB from three maize varieties and characterized their plant growth-promoting traits, including auxin production, phosphate solubilization, nitrogen fixation, and extracellular enzyme activity. Among these, Lysinibacillus sp. (ZM1) significantly enhanced root architecture, auxin levels, and nitrogen metabolism in maize seedlings, even under nitrogen-deficient conditions. Additionally, Bacillus velezensis (ZMW8) exhibited strong antifungal activity against Fusarium verticillioides and Rhizoctonia solani through lipopeptide production (bacillomycin D, fengycin), improving seedling survival and defense gene expression. Our findings address a critical gap in understanding how seed-associated microbes contribute to both growth promotion and disease protection, highlighting their potential as bioinoculants for sustainable maize cultivation. For more info see our publication.